“That case was also a primary influenza pneumonia,” Bingham continued. “Same appearance of the lung. When we looked at it histologically we were amazed at the degree of damage. It gave us a lot of respect for certain strains of influenza.”
“Seeing this case concerns me,” Jack said. “Especially in light of the other diseases that have been popping up.”
“Now, don’t head off into left field!” Bingham warned, remembering some of Jack’s comments the day before. “This isn’t out of the ordinary, like the plague case or even the tularemia. It’s flu season. Primary influenza pneumonia is a rare complication, but we see it. In fact we had a case just last month.”
Jack listened, but Bingham wasn’t making him feel any more comfortable. The patient in front of them had had a lethal infection with an agent that had the capability of spreading from patient to patient like wildfire. Jack’s only consolation was the call Laurie had made to her internist friend who’d said there were no other cases in the hospital.
“Mind if I take some washings?” Jack asked.
“Hell no!” Bingham said. “Be my guest. But be careful what you do with them.”
“Obviously,” Jack said.
Jack took the lungs over to one of the sinks, and with Vinnie’s help prepared some samples by washing out some of the small bronchioles with sterile saline. He then sterilized the outside of the containers with ether.
Jack was on his way out when Bingham asked him what he was going to do with the samples.
“Take them up to Agnes,” Jack said. “I’d like to know the subtype.”
Bingham shrugged and looked across at Calvin.
“Not a bad idea,” Calvin said.
Jack did exactly what he said he would. But he was disappointed when he presented the bottles to Agnes up on the third floor.
“We don’t have the capability of subtyping it,” she said.
“Who does?” Jack asked.
“The city or state reference lab,” Agnes said. “Or even over at the university lab. But the best place would be the CDC. They have a whole section devoted to influenza. If it were up to me, I’d send it there.”
Jack got some viral transport medium from Agnes and transferred the washings into it. Then he went up to his office. Sitting down, he placed a call to the CDC and was put through to the influenza unit. A pleasant sounding woman answered, introducing herself as Nicole Marquette.
Jack explained what he wanted, and Nicole was accommodating. She said she’d be happy to see that the influenza was typed and subtyped.
“If I manage to get the sample to you today,” Jack said, “how long would it take for you to do the typing?”
“We can’t do this overnight,” Nicole said, “if that’s what you have in mind.”
“Why not?” Jack asked impatiently.
“Well, maybe we could,” Nicole corrected herself. “If there is a sufficient vital titer in your sample, meaning enough viral particles, I suppose it is possible. Do you know what the titer is?”
“I haven’t the faintest idea,” Jack said. “But the sample was taken directly from the lung of a patient who passed away from primary influenza pneumonia. The strain is obviously virulent, and I’m worried about a possible epidemic.”
“If it is a virulent strain, then the titer might be high,” Nicole said.
“I’ll find a way to get it to you today,” Jack promised. He then gave Nicole his telephone number both at the office and at home. He told her to call anytime she had any information.
“We’ll do the best we can,” Nicole said. “But I have to warn you’ if the titer is too low it might be several weeks before I get back to you.”
“Weeks!” Jack complained. “Why?”
“Because we’ll have to grow the virus out,” Nicole explained. “We usually use ferrets, and it takes a good two weeks for an adequate antibody response which guarantees we’ll have a good harvest of virus. But once we have the virus in quantity, we can tell you a lot more than just its sub type. In fact, we can sequence its genome.”
“I’ll keep my fingers crossed that my samples have a high titer,” Jack said. “And one other question. What subtype would you think was the most virulent?”