Evolution works through mutation and selection. Mutations might occur during replication if the enzyme DNA polymerase makes a mistake. But it rarely makes a mistake. Mutations also occur because of radioactivity or ultraviolet light from the Sun or cosmic rays or chemicals in the environment, all of which can change the nucleotides or tie the nucleic acids up in knots. If the mutation rate is too high, we lose the inheritance of four billion years of painstaking evolution. If it is too low, new varieties will not be available to adapt to some future change in the environment. The evolution of life requires a more or less precise balance between mutation and selection. When that balance is achieved, remarkable adaptations occur.
A change in a single DNA nucleotide causes a change in a single amino acid in the protein for which that DNA codes. The red blood cells of people of European descent look roughly globular. The red blood cells of some people of African descent look like sickles or crescent moons. Sickle cells carry less oxygen and consequently transmit a kind of anemia. They also provide major resistance against malaria. There is no question that it is better to be anemic than to be dead. This major influence on the function of the blood – so striking as to be readily apparent in photographs of red blood cells – is the result of a change in a single nucleotide out of the ten billion in the DNA of a typical human cell. We are still ignorant of the consequences of changes in most of the other nucleotides.
We humans look rather different than a tree. Without a doubt we perceive the world differently than a tree does. But down deep, at the molecular heart of life, the trees and we are essentially identical. We both use nucleic acids for heredity; we both use proteins as enzymes to control the chemistry of our cells. Most significantly, we both use precisely the same code book for translating nucleic acid information into protein information, as do virtually all the other creatures on the planet.* The usual explanation of this molecular unity is that we are, all of us – trees and people, angler fish and slime molds and paramecia – descended from a single and common instance of the origin of life in the early history of our planet. How did the critical molecules then arise?
* The genetic code turns out to be not quite identical in all parts of all organisms on the Earth. At least a few cases are known where the transcription from DNA information into protein information in a mitochondrion employs a different code book from that used by the genes in the nucleus of the very same cell. This points to a long evolutionary separation of the genetic codes of mitochondria and nuclei, and is consistent with the idea that mitochondria were once free-living organisms incorporated into the cell in a symbiotic relationship billions of years ago. The development and emerging sophistication of that symbiosis is, incidentally, one answer to the question of what evolution was doing between the origin of the cell and the proliferation of many-celled organisms in the Cambrian explosion.
In my laboratory at Cornell University we work on, among other things, prebiological organic chemistry, making some notes of the music of life. We mix together and spark the gases of the primitive Earth: hydrogen, water, ammonia, methane, hydrogen sulfide – all present, incidentally, on the planet Jupiter today and throughout the Cosmos. The sparks correspond to lightning – also present on the ancient Earth and on modern Jupiter. The reaction vessel is initially transparent: the precursor gases are entirely invisible. But after ten minutes of sparking, we see a strange brown pigment slowly streaking the sides of the vessel. The interior gradually becomes opaque, covered with a thick brown tar. If we had used ultraviolet light – simulating the early Sun – the results would have been more or less the same. The tar is an extremely rich collection of complex organic molecules, including the constituent parts of proteins and nucleic acids. The stuff of life, it turns out, can be very easily made.
Such experiments were first performed in the early 1950’s by Stanley Miller, then a graduate student of the chemist Harold Urey. Urey had argued compellingly that the early atmosphere of the Earth was hydrogen-rich, as is most of the Cosmos; that the hydrogen has since trickled away to space from Earth, but not from massive Jupiter; and that the origin of life occurred before the hydrogen was lost. After Urey suggested that such gases be sparked, someone asked him what he expected to make in such an experiment. Urey replied, ‘Beilstein.’ Beilstein is the massive German compendium in 28 volumes, listing all the organic molecules known to chemists.
Using only the most abundant gases that were present on the early Earth and almost any energy source that breaks chemical bonds, we can produce the essential building blocks of life. But in our vessel are only the notes of the music of life – not the music itself. The molecular building blocks must be put together in the correct sequence. Life is certainly more than the amino acids that make up its proteins and the nucleotides that make up its nucleic acids. But even in ordering these building blocks into long-chain molecules, there has been substantial laboratory progress. Amino acids have been assembled under primitive Earth conditions into molecules resembling proteins. Some of them feebly control useful chemical reactions, as enzymes do. Nucleotides have been put together into strands of nucleic acid a few dozen units long. Under the right circumstances in the test tube, short nucleic acids can synthesize identical copies of themselves.
No one has so far mixed together the gases and waters of the primitive Earth and at the end of the experiment had something crawl out of the test tube. The smallest living things known, the viroids, are composed of less than 10,000 atoms. They cause several different diseases in cultivated plants and have probably most recently evolved from more complex organisms rather than from simpler ones. Indeed, it is hard to imagine a still simpler organism that is in any sense alive. Viroids are composed exclusively of nucleic acid, unlike the viruses, which also have a protein coat. They are no more than a single strand of RNA with either a linear or a closed circular geometry. Viroids can be so small and still thrive because they are thoroughgoing, unremitting parasites. Like viruses, they simply take over the molecular machinery of a much larger, well-functioning cell and change it from a factory for making more cells into a factory for making more viroids.
The smallest known free-living organisms are the PPLO (pleuropneumonia-like organisms) and similar small beasts. They are composed of about fifty million atoms. Such organisms, having to be more self-reliant, are also more complicated than viroids and viruses. But the environment of the Earth today is not extremely favorable for simple forms of life. You have to work hard to make a living. You have to be careful about predators. In the early history of our planet, however, when enormous amounts of organic molecules were being produced by sunlight in a hydrogen-rich atmosphere, very simple, nonparasitic organisms had a fighting chance. The first living things may have been something like free-living viroids only a few hundred nucleotides long. Experimental work on making such creatures from scratch may begin by the end of the century. There is still much to be understood about the origin of life, including the origin of the genetic code. But we have been performing such experiments for only some thirty years. Nature has had a four-billion-year head start. All in all, we have not done badly.
Nothing in such experiments is unique to the Earth. The initial gases, and the energy sources, are common throughout the Cosmos. Chemical reactions like those in our laboratory vessels may be responsible for the organic matter in interstellar space and the amino acids found in meteorites. Some similar chemistry must have occurred on a billion other worlds in the Milky Way Galaxy. The molecules of life fill the Cosmos.
But even if life on another planet has the same molecular chemistry as life here, there is no reason to expect it to resemble familiar organisms. Consider the enormous diversity of living things on Earth, all of which share the same planet and an identical molecular biology. Those other beasts and vegetables are probably radically different from any organism we know here. There may be some convergent evolution because there may be only one best solution to a certain environmental problem – something like two eyes, for example, for binocular vision at optical frequencies. But in general the random character of the evolutionary process should create extraterrestrial creatures very different from any that we know.
I cannot tell you what an extraterrestrial being would look like. I am terribly limited by the fact that I know only one kind of life, life on Earth. Some people – science fiction writers and artists, for instance – have speculated on what other beings might be like. I am skeptical about most of those extraterrestrial visions. They seem to me to rely too much on forms of life we already know. Any given organism is the way it is because of a long series of individually unlikely steps. I do not think life anywhere else would look very much like a reptile, or an insect or a human – even with such minor cosmetic adjustments as green skin, pointy ears and antennae. But if you pressed me, I could try to imagine something rather different.